In this example we have a set of images that contain clusters of bacteria cells. Running an automated counter without enhancing the images will cause each cluster to be counted as a single cell. However, we want to enumerate each individual cell. We use a combination of filters to separate the cells within each cluster, allowing Skipper to detect all individual cells.

Filters used

The set of filters used in the above example includes a Marr-Hildreth convolution filter, grayscale filter, and a threshold filter.

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